ASTM D – 15 Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified. RRIM (MS P) and the ASTM (D ) modified Lowry tests, were examined and their relationship studied. Total extractable proteins, EPRRiMt. ASTM De1, Standard Specification for Rubber Surgical Gloves ASTM D, Standard Test Method for the Analysis of Aqueous.

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D57712 displacement, the protein become mobile and easier to dispose off. Are you sure you want to empty the cart? In most cases the powder is removed by washing with water which further reduces the protein content by extraction.

Leap latex elisa for antigenic proteins, Volume 61, No. Some laboratories claim that they can measure lower levels of proteins.

ASTM International – Standard References for ASTM D – 15

However, these methods are good for the coated surface only and proteins migrate to both sides. Introduction The problem of proteins in natural rubber Type I latex allergies can be caused by proteins in the natural latex used in gloves, condoms, balloons and other articles. It then could be concluded that the latex has been cleaned.

According to the literature,8 when the latex is in the tree, the particles are presumed to possess an adsorbed layer of proteins—lipid complex. The silica dipping process is simple, short and inexpensive. Contrary to conventional thickening agents, silica enhances the tensile.

ASTM D – Click here to download full list of books. Historically latex gloves are powdered for two reasons. Experimental results and discussion Low-protein allergen analysis in latex compounded with fumed silica Compounding of fumed silica dispersion for dipped natural latex application resulted in tensile and tear enhancement as described before.


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The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard. Further analysis by the LEAP method also at Guthrie Research, resulted in clean films having protein values below the detection level, 0. Powder-free latex gloves and other methods of reducing protein content Latex gloves are among the most commonly used critical latex articles. This paper suggests a way to eliminate the proteins with a simple, elegant and inexpensive method using fumed silica additives.

Total and specific analysis of proteins in latex goods The total quantity of latex proteins in gloves is evaluated by extracting the rubber with an aqueous solution and measuring according to ASTM D Latex protein analysis ASTM D, might not represent a true picture of the latex irritation problem. A non-silica rubber blank has micrograms of proteins per gram of rubber, see Table I.

The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.

Washing is minimal and since the latex is clean, there is no need for costly off line post treatments. Print 1 2 3 4 5 page sstarting from page current page. Thompson et al9 that there is a relationship between hydration and compromised barriers in some surgical gloves. November 20, Content source: As a consequence of the addition of ammonia for preserving the latex concentrate, the lipid materials are believed to hydrolyze slowly, releasing fatty acids.


It is suggested that the silica attaches itself to the surface of the latex particle substituting the protein molecules and displacing them, see Fig. An additional advantage is enhancing latex viscosity resulted only with fumed silica B. Test Method A definitive procedure that produces a test result: Other methods for protein removal treatment include passing the latex through special ion exchange resins6 or altering the protein structure with protease enzymes.

For example, someone can develop a process for latex protein cleaning, submit the clean latex to ASTM D test and find that the protein level was reduced from ppm to 20 ppm.

A definitive procedure that produces a test result: However, the low silica concentrations necessary make this a cost-effective process for addressing protein allergy concerns. FORMAT Single User Subscription Site License Subscription BOOKS All Books PACKAGES All Packages EDITION to PUBLISHER. Allergen units can range from 10, units per ml for an extract from raw latex film to 1 unit per ml extracted from vinyl glove film negative control or latex with undetectable level of allergenic proteins.

ASTM D5712 – 15

Some of the proteins can be more harmful than others. Enter your account email address to request a password reset: However, traces still may remain and cause skin reactions.

Navigation Top Stories Search. The silica also may serve as a thickening agent.