年1月23日 CalcuSyn is a software tool whose main purpose is to aid individuals in analyzing mixed drug treatments, a more and more used practice in. combination-index data generated by CalcuSyn software analyses .. and antagonism, respectively (Source: CalcuSyn manual, Biosoft, ). Manual and Software, Biosoft, Cambridge, U.K., Chou, T.-C. and Hayball , M. CalcuSyn for Windows, Multiple-drug dose-effect analyzer and manual.
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Cisplatin Data points entered: These ambiguous practices manial confusion. Paclitaxel Data points entered: The large majority of combination regimens are being developed empirically manusl there are few experimental studies designed to explore thoroughly calcusny drug combinations, using appropriate methods of analysis.
In conclusion, CalcuSyn is an efficient piece of software which can help people in creating new mixed drug treatment and more effective ones. This terminology was formerly introduced by J. Reports You can export report data to a text.
Constant Ratio Combination Design The most efficient way for experimental design is to choose the combination drugs at their equipotent ratio e. For single drugs, this will be the median plot and the dose-effect plot. Click OK to complete the entry. The algebraic estimation algorithm makes the assumption that the uncertainty in the dose measurement is normally distributed.
CalcuSyn automatically graphs the data and produces reports giving summary statistics on all drugs plus detailed analysis of drug interactions including the Combination Index and EDx for any value of x.
CalcuSyn 22 Step 3 – Now specify the drug data by clicking the Select button. However, the Dx-normalized isobologam can be constructed automatically.
Topotecan Data points entered: There is no easy way to answer the synergy question.
The report window will be displayed – this is a standard text window with most of the controls available in Notepad. It is possible to generate isobolograms for three drug combinations as described by Chou and Callcusyn 14, Note that the CD version has Autorun and, if your computer is suitable, that will present a menu on-screen from which callcusyn can select the Install option.
Step 4 – Select the drugs from the list, ensuring that constant ratio is ticked and then enter the ratio and click OK. If any CI value s are greater than 2, it is recommended that the antilog of the averaged log CI value is used to avoid significant biases of overestimating antagonism. The n can be partitioned into any combination of two parts which can be assigned to the two coordinates of the isobol 8.
Registration The first time you run CalcuSyn, you will be presented with a dialog containing a serial number and requesting maunal password.
If the Monte Carlo option is selected in the Reporting Options dialog then the mean and standard deviation values of CI calculated using the Monte Carlo algorithm are displayed as for the CI simulations above.
You can only import data from the first sheet of a workbook. Two drug combinations, if used, should be correspond to three drug ratios so that D1: MTX Fraction affected uM 0. First and foremost, you should know it is possible to import data from TXT and XLS files, as well as export it to such documents, so that you can use it in other programs or keep it safe.
Select the data source using the standard windows dialog and click OK.
Welcome to COMPUSYN
The following table shows the recommended symbols and descriptions for presenting the ,anual of synergism or antagonism. Using the Program 55 Data points entered: It is a rigorous and well developed discipline.
It can make multiple calculations using the Median Effect methods. You will then be required to enter the dose data for all drugs in your combination. The general isobologram equation for the n drug combination has been derived by Chou and Talalay 8,9.
As long as the m and Dm parameters for each single drug are available, the CI values for each data point of the nonconstant ratio design can still be calculated.
Using the Program 47 Data points entered: The second contains the effects that correspond to each dose effect1 to n. The drug concentrations of two drugs on the coordinates of the isobol are normalized by the corresponding EDx doses or concentrations. Multi-drug combinations greater than 2 follow a similar equation but cannot be performed for the mutually non-exclusive case.
The plots drawn by CalcuSyn include dose-effect, median-effect, isobolograms and CI-effect. Three-dimensional isobols for three drug combinations: The approximated error in CI for the mutually exclusive case now becomes: Graphs and the data used to plot them can be copied to the clipboard and exported to file from the right-click menu.
CalcuSyn is a software tool whose main purpose is to aid individuals in analyzing mixed drug treatments, a more and more used practice in medicine.
A powerful and straightforward combined drug effects analyzer which also enables you to import and export information to various file formats. It can also be extended to multiple ratios e. The ratio of drugs in combinations has to be constant across doses. Three data files are installed with CalcuSyn in its Samples folder.
The pitfalls of synergy interpretation are described in Ref.
This is the first drug Data points entered: ADP-ribose Fraction affected uM 95 0.